[Histonet] in vitro skin Fb staining w/vimentin

Katri Tuomala katri <@t> cogeco.ca
Fri Jan 27 21:32:14 CST 2006

Hi Birthe,
Vimentin is not specific marker for fibroblasts, although normal epithelial 
cells do not usually have that intermediate filament present. Many other 
cells do stain positively for vimentin, for instance melanocytes, 
macrophages and all lymphoid cells. Those are all present in the skin.

Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Birthe Schnegelsberg" <schnegelsberg <@t> xgene.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Sunday, February 26, 2006 11:29 AM
Subject: [Histonet] in vitro skin Fb staining w/vimentin

> Hello.
> I am staining cells in in vitro skin with the fibroblast specific marker
> vimentin to differentiate between fibroblasts and keratinocytes.
> I am able to detect fluorescent signals in the tissue. The staining 
> pattern
> and the cell localization makes me wonder though, if the staining is in 
> fact
> specific fibroblast staining or if the antibody (immunon) picks up dead 
> cell
> material.
> Does anybody have experienece using vimentin as a marker for fibroblast
> cells?
> The staining pattern of the positive control looks different than that for
> the singel cells in the in vitro skin?!
> Thanks, Birthe
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