[Histonet] fixation of rat cerebellum

[Johns, Laura [CNTUS]]

I am looking for astrocytes. 

Thanks,
Laura

-----Original Message-----
From: Favara, Cynthia (NIH/NIAID) [E] [mailto:cfavara <@t> niaid.nih.gov]
Sent: Thursday, January 26, 2006 4:13 PM
To: Johns, Laura [CNTUS]
Subject: RE: [Histonet] fixation of rat cerebellum


It may depend on what you are looking for. I routinely keep
mouse/hamster brain for 7-10 days in NBF. We work with prions and do a
number of IHC stains with no difficulties.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Johns, Laura [CNTUS] [mailto:LJohns53 <@t> CNTUS.JNJ.COM] 
Sent: Thursday, January 26, 2006 1:15 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] fixation of rat cerebellum

Hi All,

Does anyone have a suggestion for the length of time needed for fixation
of
rat cerebellum?  We want to perform immunohistochemistry.

Thanks,
Laura

Laura M. Johns, Ph.D.
Centocor, Inc.
145 King of Prussia Road (Mail Stop # R-4-2)
Radnor, PA 19087
Phone:  610-240-8284 ....  Fax:  610-240-8150....  Email:
ljohns53 <@t> cntus.jnj.com

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