[Histonet] fixation of rat cerebellum

Johns, Laura [CNTUS] LJohns53 <@t> CNTUS.JNJ.COM
Thu Jan 26 15:16:35 CST 2006

I am looking for astrocytes. 


-----Original Message-----
From: Favara, Cynthia (NIH/NIAID) [E] [mailto:cfavara <@t> niaid.nih.gov]
Sent: Thursday, January 26, 2006 4:13 PM
To: Johns, Laura [CNTUS]
Subject: RE: [Histonet] fixation of rat cerebellum

It may depend on what you are looking for. I routinely keep
mouse/hamster brain for 7-10 days in NBF. We work with prions and do a
number of IHC stains with no difficulties.


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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-----Original Message-----
From: Johns, Laura [CNTUS] [mailto:LJohns53 <@t> CNTUS.JNJ.COM] 
Sent: Thursday, January 26, 2006 1:15 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] fixation of rat cerebellum

Hi All,

Does anyone have a suggestion for the length of time needed for fixation
rat cerebellum?  We want to perform immunohistochemistry.


Laura M. Johns, Ph.D.
Centocor, Inc.
145 King of Prussia Road (Mail Stop # R-4-2)
Radnor, PA 19087
Phone:  610-240-8284 ....  Fax:  610-240-8150....  Email:
ljohns53 <@t> cntus.jnj.com

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