[Histonet] Counter stain
bjdewe <@t> aol.com
bjdewe <@t> aol.com
Fri Jan 20 12:33:44 CST 2006
>>or not use any counterstain at all since the antigenic site is so small
Yes, that is what I've done but they would be sooo pretty to photograph with a background stain...
Cheers,
Lorie
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-----Original Message-----
From: Gayle Callis <gcallis <@t> montana.edu>
To: bjdewe <@t> aol.com
Sent: Fri, 20 Jan 2006 08:30:59 -0700
Subject: Re: [Histonet] Counter stain
Eosin? or not use any counterstain at all since the antigenic site is so small, hard to visualize after hematoxylin.
At 11:28 AM 1/19/2006, you wrote:
>I am doing a peroxidase DAB stain for an antigen that is present only in >small quantities in the nucleus. I am doing it on rat bone. If I >counterstain with hematoxylin it covers the DAB stain. What else can I use >that will only stain the cytoplasm and not the nucleus. i thought of >methyl green but it too is supposed to stain the nucleus. I thought of >neutral red but it won't look so good with the brown. Suggestions?
>
>Cheers,
>Lorie
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
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