[Histonet] Invertebrates in Sediment

[John Kiernan]

Rose Bengal B is an anionic, lipophilic xanthene
dye, related to the eosins and erythrosins. One of
its documented uses is staining foraminifera in
marine sediments. Get your boss to send you to the
library to check out "Conn's Biological Stains"
and Clark's "Staining Procedures" for references
to the original methods. If it's a specialized
application, go to the references cited in these
books. 

John Kiernan
London, Canada.
______________________________________________________
BennettW <@t> pac.dfo-mpo.gc.ca wrote:
> 
> Hello Histonetters,
> 
> Thanks in advance for spending any time with this query!  I have a
> researcher that has collected sediment samples, preserved with NBF, and
> wants to stain any invertebrates in the sediment so that they can be
> enumerated and identified.  The researcher wants to use rose bengal but
> doesn't know at what concentration and neither do I.  Can anyone help?
> 
> Thanks
> Bill Bennett
> Histologist
> Fisheries and Oceans Canada
> Pacific Biological Station
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