[Histonet] Counter stain

Connolly, Brett M brett_connolly <@t> merck.com
Thu Jan 19 12:40:32 CST 2006


I've used 0.25% Metanil Yellow and/or Light Green...either one works fine,
just play with the times to get the staining intensity you want.


Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803
e-mail. brett_connolly <@t> merck.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
bjdewe <@t> aol.com
Sent: Thursday, January 19, 2006 1:29 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Counter stain

I am doing a peroxidase DAB stain for an antigen that is present only in
small quantities in the nucleus. I am doing it on rat bone. If I
counterstain with hematoxylin it covers the DAB stain. What else can I use
that will only stain the cytoplasm and not the nucleus. i thought of methyl
green but it too is supposed to stain the nucleus. I thought of neutral red
but it won't look so good with the brown. Suggestions?
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