[Histonet] antigen retrieval on cryosections

[Till, Renee]

Any helpful hints for keeping cryosections on the slide during antigen
retrieval? I am not very experience in cryosections used for immunos,
but they have cd markers that have to be antigen retrieved. Using the
same retrieval as I do however often makes the sections come off the
slides. I believe they use just Superfrost Plus slides. Would any other
type of slide help? Right now they use Citra Plus in the microwave for 2
minutes on power 10, then 10 minutes on power 1. That is what I do for
my paraffin sections and it is fine. Perhaps less time? Or even a
different antigen retrieval solution? I have asked for help with
problems before that this lab has had. They always hire techs for
everthing else and want me to teach them the histo part of it. 

 


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