[Histonet] RE: Eosin too pink
JMacDonald <@t> mtsac.edu
Tue Jan 17 23:28:55 CST 2006
Eosin is differentiated in the dehydrating alcohols. You ca the time in the alcohols to get a lighter pink. Also contact tech support at Richard-Allan. There are two types of eosin that they sell and one is really concentrated.
-----histonet-bounces <@t> lists.utsouthwestern.edu wrote: ---
To: From: Mark Adam Tarango <marktarang Sent by: histonet-bounces <@t> lists.utsouthwestern.eduDate: 01/14/2006
Subject: [Histonet] RE: Eosin too pink
pink? I after Eosin or if pinker....it's more orange when alcohol in the alcohol after eosin(just see anyway). If it's just too strong, dilute it down wi I hardly get any specimens, so I don't like changing the often, i just keep adding alcohol to it when it gets low and
crusted Mark T.
&g > To: histonet <@t> lists.utsou > Subject: [Histonet] Eosin too pink
> H > We are currently doing our H & E stain by hand an > Richard-Allan 7211 Hematoxylin and their Eosin Y. pathologist is
> still complaining of slides being TOO PINK. &nb the eosin step
> down to just 5 dips from 15 dips, and Eosin is very
> Thi > Hydrate to water like normal (thru Clear Rite 3, Flex)
> Hematoxylin- 2 mins
> Rinsing in water u > Clarifier II- 30 dips
> running wat > Richard Allan Bluing - 30 dips
> running water - > 95% Flex - 10 dips
> Eosin-Y- 5 quick dips
> Any suggestions on a pos Eosin.
> Complaint seems to solely experimenting w/
> cutting the eo but, the
> Hemo looks if that
> will test slides.
> Helayne Parker
> Histology Sectio > Skaggs Community Health Center
> Branson, Missouri
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