[Histonet] RE: Eosin too pink

[Jennifer MacDonald]

   Eosin is differentiated in the dehydrating alcohols.  You ca   the  time  in  the  alcohols to get a lighter pink.  Also contact   tech  support  at  Richard-Allan.   There are two types of eosin that   they sell and one is really concentrated.

   Jennifer

   -----histonet-bounces <@t> lists.utsouthwestern.edu wrote: ---
     To:      From: Mark Adam Tarango <marktarang     Sent  by: histonet-bounces <@t> lists.utsouthwestern.eduDate: 01/14/2006
     06:03PM
     Subject: [Histonet] RE: Eosin too pink
          pink?  I     after  Eosin  or  if     pinker....it's  more  orange when      alcohol  in  the  alcohol  after  eosin(just see     anyway).   If  it's just too strong, dilute it down wi     I  hardly  get  any specimens, so I don't like changing the      often,  i  just  keep  adding  alcohol  to  it when it gets low and
     crusted      Mark T.
     &g     > To: histonet <@t> lists.utsou     > Subject: [Histonet] Eosin too pink
     >
     > H     >  We are currently doing our H & E stain by hand an     >  Richard-Allan  7211  Hematoxylin  and  their  Eosin  Y.        pathologist is
     >  still  complaining  of slides being TOO PINK. &nb     the eosin step
     >  down  to  just  5  dips from 15 dips, and      Eosin is very
     > rich.
     >
     > Thi     >  Hydrate  to  water like normal (thru Clear Rite 3,     Flex)
     > Hematoxylin- 2 mins
     > Rinsing in water u     > Clarifier II- 30 dips
     > running wat     > Richard Allan Bluing - 30 dips
     > running water -      > 95% Flex - 10 dips
     > Eosin-Y- 5 quick dips
          >
     >  Any  suggestions  on  a  pos     Eosin.
     >  Complaint  seems  to  solely     experimenting w/
     >  cutting  the eo     but, the
     >  Hemo looks     if that
     >  will       test slides.
     >     >
     > Helayne Parker
     > Histology Sectio     > Skaggs Community Health Center
     > Branson, Missouri
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