[Histonet] fixation

[Kim O'Sullivan]

Hi all,

Have recieved some mouse kidneys recently to work on which have been fixed in PLP for aproximatley 2 hours and 7% sucrose for 2 days afterwards (5 changes), but the glomeruli appear shrunken and the bowmans space enlarged (not a product of the disease we are researching). Is this caused by overfixing or underfixing?

Alternatively (as this method is quite common and we have not had this problem in the past) could this result occur throught the dehyration or rehydration steps during the staining process?

Any ideas would be helpful

Kim O'Sullivan