[Histonet] Eosin too pink

Bryan Llewellyn llewllew <@t> shaw.ca
Fri Jan 13 14:25:56 CST 2006

There have been many suggestions made so far, all valid.  Here's another 
one.  Try making a 0.1% solution of eosin Y in 95% ethanol, stain for 15 
seconds with 2 seconds agitation at the beginning, then rinse thoroughly 
with gentle agitation in 95% ethanol for about 10 seconds just to remove the 
excess eosin solution (i.e. use it progressively).  If still too pink, 
dilute in half and repeat.  Do this until you have the required density. 
Cutting the staining time to 5 dips from 15 dips is pretty drastic (what 
kind of dips - how long is each dip?).  Very short staining times are not 
usually the best, as has been said, so I suggest you keep the time standard 
and adjust the concentration.  The best way to solve this kind of problem is 
always to run a systematic series of trials using sections of your common 
tissues with different concentrations of dye and times of application.

Bryan Llewellyn 

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