[Histonet] Eosin too pink
llewllew <@t> shaw.ca
Fri Jan 13 14:25:56 CST 2006
There have been many suggestions made so far, all valid. Here's another
one. Try making a 0.1% solution of eosin Y in 95% ethanol, stain for 15
seconds with 2 seconds agitation at the beginning, then rinse thoroughly
with gentle agitation in 95% ethanol for about 10 seconds just to remove the
excess eosin solution (i.e. use it progressively). If still too pink,
dilute in half and repeat. Do this until you have the required density.
Cutting the staining time to 5 dips from 15 dips is pretty drastic (what
kind of dips - how long is each dip?). Very short staining times are not
usually the best, as has been said, so I suggest you keep the time standard
and adjust the concentration. The best way to solve this kind of problem is
always to run a systematic series of trials using sections of your common
tissues with different concentrations of dye and times of application.
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