[Histonet] antibodies to distinguish neurons and glia

Caroline Bass cbass <@t> bidmc.harvard.edu
Thu Jan 12 20:16:25 CST 2006


Hi Guys,

I have  a protein being expressed in the brain and I would like to  
see if it is in neurons or glial cells.  Could someone recommend some  
good antibodies for this?  I think GFAP and NeuN are considered the  
standard.

I just need a reliable source for these.  If you have any suggestions  
as to dilutions and incubations times that would be great.

This is paraformaldehyde fixed mouse brain, 30 micron floating  
sections.  I am planning to do double fluorescent immunostaining.

Thanks,

Caroline Bass 



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