[Histonet] antibodies to distinguish neurons and glia
Caroline Bass
cbass <@t> bidmc.harvard.edu
Thu Jan 12 20:16:25 CST 2006
Hi Guys,
I have a protein being expressed in the brain and I would like to
see if it is in neurons or glial cells. Could someone recommend some
good antibodies for this? I think GFAP and NeuN are considered the
standard.
I just need a reliable source for these. If you have any suggestions
as to dilutions and incubations times that would be great.
This is paraformaldehyde fixed mouse brain, 30 micron floating
sections. I am planning to do double fluorescent immunostaining.
Thanks,
Caroline Bass
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