[Histonet] non-specific cross reactivity with mucus
wilson_jm <@t> cimar.org
Thu Jan 12 19:05:25 CST 2006
I work with fish in a resarch lab and with some of the antibodies I use
I get what I would consider non-specific crossreactivity with mucus either
covering the body surface or in mucocyte granules. More often this problem
would arise with rabbit polyclonal anti-peptide antibodies using neat serum.
I usually get around this by affinity purifying using the peptide but in
some cases this is not possible.
Someone once told me that mucoproteins have a large number of antigenic
determinants increasing the likelyhood of crossreactivity but I could never
find a reference. I've searched through the archives but couldn't find an
explanation except for a suggestion that it might be charge related. Could
someone please shed some light on this situation for me. It is something
that has bugged me for the longest time.
Thank you for any insight.
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