[Histonet] improper fixation

Rittman, Barry R Barry.R.Rittman <@t> uth.tmc.edu
Wed Jan 11 08:36:35 CST 2006

While you can do this, the damage has been done and it is unlikely that
you will achieve a better fixation. Also in going back through xylenes
and alcohols you will probably cause greater mechanical damage. 
Even though you processed the tissues, a certain amount of fixation or
post fixation has occurred in the alcohols used in processing.
Might I suggest that you could try to mordant the sections after they
have been deparaffinized and bring down to water? Can use Bouin's or a
variety of other solutions that may improve the staining for you.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kristopher Kalleberg
Sent: Wednesday, January 11, 2006 8:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] improper fixation


Last week I was unable to collect 18 biopsies 2mm biopsies for a
clinical that
is being run and had a colleague collect them for me.  After running
H&E and Fontana Mason stains I found that two biopsies were improperly
Since I have never had this problem before, I was wondering if it is
to run the sample backwards through paraffin, xylenes, and alcohols and
fix the samples again in 10% NBF and reprocess to paraffin.  If this is
possible doe sanyone have a protocol or know what the effects will be by
this?  Thank you in advance.

Kris Kalleberg
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