[Histonet] improper fixation

Kristopher Kalleberg Kristopher.Kalleberg <@t> unilever.com
Wed Jan 11 08:16:13 CST 2006


Last week I was unable to collect 18 biopsies 2mm biopsies for a clinical that
is being run and had a colleague collect them for me.  After running routine
H&E and Fontana Mason stains I found that two biopsies were improperly fixed. 
Since I have never had this problem before, I was wondering if it is possible
to run the sample backwards through paraffin, xylenes, and alcohols and then
fix the samples again in 10% NBF and reprocess to paraffin.  If this is
possible doe sanyone have a protocol or know what the effects will be by doing
this?  Thank you in advance.

Kris Kalleberg

More information about the Histonet mailing list