[Histonet] Re: Reihard, please help CD4, CD8 in FFPE Bone Marrow

[Kuo Christy]

I've treated the H2O2 step.
However, my background is very high.
I was wondering if you treated any other step and your antibody
incubation time and concentration.

Thank you in advance for answering~


sincerely,
Christy

On 1/11/06, Lori Richey <lrichey <@t> u.washington.edu> wrote:
> We use both CD4 and CD8 on FFPE sections.  We had problems with the CD4
> until we changed our quenching step to 0.5% H2O2 in MEOH for 10 min. The
> pretreatment for both is 15 min microwave in pH 8 EDTA.
>
> Thomas Pier wrote:
>
> >Hello,
> >I am looking for Reinhard von Wasielewski.  I recently saw a post
> >stating that he had working protocols for CD4 and CD8 that even worked
> >in decalcified bone marrow.  CD4 and CD8 in FFPE, decalcified bone
> >marrow is exactly what I'm having problems with.  I have been banging my
> >head against the wall with this for some time now.  I would really
> >appreciate any help I could get on this.  Thank you very much.
> >
> >Tom Pier
> >
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