[Histonet] Reihard, please help CD4, CD8 in FFPE Bone Marrow
lrichey <@t> u.washington.edu
Tue Jan 10 17:02:33 CST 2006
We use both CD4 and CD8 on FFPE sections. We had problems with the CD4
until we changed our quenching step to 0.5% H2O2 in MEOH for 10 min. The
pretreatment for both is 15 min microwave in pH 8 EDTA.
Thomas Pier wrote:
>I am looking for Reinhard von Wasielewski. I recently saw a post
>stating that he had working protocols for CD4 and CD8 that even worked
>in decalcified bone marrow. CD4 and CD8 in FFPE, decalcified bone
>marrow is exactly what I'm having problems with. I have been banging my
>head against the wall with this for some time now. I would really
>appreciate any help I could get on this. Thank you very much.
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