[Histonet] Slide Press?

[Rittman, Barry R]

Amy
We had a similar problem with ground sections and also sheets of
epithelium from mouse ears.
Our solution was to use weights made by cutting solid brass cylinders
(3/4" diameter and 2" long) and placing these weights over the area
under the cover glass where section was located. This worked very well
for us even if sections were slightly wedge shaped. This also allows you
to add additional mountant if air tends to creep in.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Tuesday, January 10, 2006 1:56 PM
To: Amy Porter; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide Press?

We placed are slides between sheets of polyethylene (cut from a thicker 
baggie) with a sheet between each slide, then clamped the slides between

pieces of wood so as to NOT break the glass slides, using C clamps from
a 
hardward store to apply pressure evenly. Clamps come in all sizes, but
one 
can find them at Walmart too.

You can be as creative on this as you want - something cheap, fast and 
efficient.

At 12:20 PM 1/10/2006, you wrote:
>I have had a question posed to me by our e.m. technician about a "slide

>press" to weigh down section of bone that have been embedded in 
>resin.  Does anyone out there know what this is or know of a vendor
where 
>one may be purchased.  Thanks in advance for all of the responses.
>
>Amy S. Porter, HT(ASCP) QIHC - Supervisor
>Michigan State University
>Laboratory for Investigative HistoPathology
>Department of Physiology / Division of Human Pathology
>2100 Biomedical Physical Sciences Bldg. Room #2133
>East Lansing, MI  48824-3320
>Phone:  (517) 355-6475 ext 1480 / Fax:  (517) 432-1368
>Email:  portera <@t> msu.edu
>www.humanpathology.msu.edu
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet