[Histonet] Microwave slide drying vs. conventional slide dryi ng

[Smith, Allen]

I have always left my slides on a 50 degree warming plate for 48 hours.  The
slides are dry in an hour, but sections will fall off during a PAS reaction.
After 48 hr I rarely lose a section no matter what I do to it.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kinsley,
David
Sent: Friday, January 06, 2006 2:57 PM
To: 'Laurie Colbert'; lharris <@t> samhealth.org; Histonet (E-mail)
Subject: RE: [Histonet] Microwave slide drying vs. conventional slide dryi
ng

Can you let me know what tissues samples and temperature you typically use
with the hot plate method?  

I work with animal tissues and usually place my slides on a hot plate at
about 45-50 degrees to flatten the sections out (about 1 hour) and then
follow by heating them in a 60 degree oven for 4 hours to overnight
depending on the sample. 

Dave


-----Original Message-----
From: Laurie Colbert [mailto:laurie.colbert <@t> huntingtonhospital.com] 
Sent: Friday, January 06, 2006 12:38 PM
To: lharris <@t> samhealth.org; Histonet (E-mail)
Subject: RE: [Histonet] Microwave slide drying vs. conventional slide drying


Lori,

We don't use either.  We simply put our drained slides on the hot plate for
about 5 minutes.  In previous jobs, we always used an oven, and when I came
here I was really nervous about just using the hot plate - but it works!

Laurie Colbert
Huntington Hospital
Pasadena, CA

-----Original Message-----
From: lharris <@t> samhealth.org [mailto:lharris <@t> samhealth.org]
Sent: Friday, January 06, 2006 8:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave slide drying vs. conventional slide drying


We are having a discussion about slide drying at out facility. How many of
you out there use a microwave to dry your H&E slides before staining versus
using a conventional 70 degree Celsius hot air slide drying oven for 20
minutes? I personally against microwave slide drying but the doctors like it
because it takes less time (3 min. 30 sec. on medium setting). Thanks for
your response.

Lori A. Harris, HT (ASCP)
Histology Section Leader
GSRMC - Pathology
3600 NW Samaritan Drive
Corvallis, OR 97330
1-541-768-6078
lharris <@t> samhealth.org




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