[Histonet] Bone marrow aspirate smears

Rene J Buesa rjbuesa <@t> yahoo.com
Sun Jan 8 07:43:32 CST 2006

Hi Kari:
  In our hospital the residents sample the bone marrow and prepare the smears in site and place them in regular slides folders.The aspirates are fixed in neutral buffered formalin and both are brought to the histology lab. 
  The smears (already air dried ) are fixed with methanol as any blood smear and 2 are stained (Wright-Giemsa and Perl's for iron) and the rest saved for any further test (like IHC). 
  The aspirates are filtered and a block is prepared for histology. The small bone fragment is decalcified with EDTA (only) for 4-6 hours, and processed afterwards.
  This protocol has worked well for over 20 years.
  Hope this will help!
  René J.

Katri Tuomala <katri <@t> cogeco.ca> wrote:
  Hi Histonetters,
This is a bit outside of histology, but I'll try for help anyway. A research 
colleague of mine is wondering, how to best collect bone marrow aspirates 
for making smears or cytospins for immunocytochemistry. What if the marrow 
collection is done in one place and have to be transported to place of 
testing? Any ideas, who would have general information about this type of 

Katri Tuomala
Hamilton, Ontario, Canada 

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