[Histonet] Processing/embedding question - your opinion

[LuAnn Anderson]

Why not do a comparison--take two pieces of the 
same tissue. Process both-embed one immediately 
and delay the second. Cut, stain and compare morphology.
LuAnn


At 11:54 AM 1/6/2006, Trajkovic, Dusko wrote:
>I have yet to see any published information 
>stating that un-embedded tissue left to 
>solidify, is damaging in any way or form. My 
>colleagues an I in research, have done it 
>numerous times, for various reasons, non were for being lazy.
>
>Dusko Trajkovic
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu 
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
>Sent: Friday, January 06, 2006 9:43 AM
>To: Bonnie Whitaker; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Processing/embedding question - your opinion
>
>Bonnie:
>   That is my personal and very particular 
> opinion, and I think I am entitled to that,
>   in the same way that you have expressed yours.
>   I believe that this is covered by the freedom of expression, is it not?
>   René J.
>
>Bonnie Whitaker <bwhitaker <@t> brownpathology.com> wrote:
>   Rene,
>
>What is "lazy and indolent" about saving processed tissue without embedding
>it? I frequently collect many tissues for embedding in multi-tissue blocks,
>and I see no point in embedding the tissue temporarily while I continue to
>collect additional material. It makes no difference if the paraffin coating
>is minimal or a full block. You can also store a lot more control tissue in
>a small area if it is maintained in unembedded cassettes. One cassette can
>hold several blocks worth of material.
>
>Bonnie Whitaker
>Lab Manager
>Brown & Associates Medical Laboratories
>8076 El Rio
>Houston, Texas 77054
>713-741-6677
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
>Sent: Friday, January 06, 2006 10:58 AM
>To: foley1; histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Processing/embedding question - your opinion
>
>
>Hi Foley:
>I have seen that done in several places. I have also seen all the
>cassettes wrapped in aluminum foil and kept that way (like a pizza
>left-over).
>I cannot tell you what would be the consequences for the morphology or
>future tests.
>Theoretically speaking probably this practice will not affect because the
>tissue itself and all its components are supposedly embedded in paraffin
>that will just solidify.
>I personally do not like this to be done. For me, personally, it indicates
>laziness, indolence and even "disrespect" for the tissue sample.
>Once I was confronted with the need of keeping processed cassettes in a
>secure way before casting the blocks. My solution was to put all the
>cassettes in a shallow
>plastic container, place all the blocks in it, add melted paraffin and
>prepare one single block, as large as the container. When I was able to
>prepare the blocks individually, I melted the paraffin and prepared the
>blocks.
>Hope this will help you!
>René J.
>
>foley1 wrote:
>Does anyone routinely allow for the hot wax to drain off multiple
>cassettes of processed tissue and be held at room temperature for multiple
>days (6
>days) before embedding? What would the consequences be to morphology,
>possible immunohistochemistry and molecular (DNA) studies?
>
>
>
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