[Histonet] Processing/embedding question - your opinion

[Trajkovic, Dusko]

I have yet to see any published information stating that un-embedded tissue left to solidify, is damaging in any way or form. My colleagues an I in research, have done it numerous times, for various reasons, non were for being lazy.

Dusko Trajkovic


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, January 06, 2006 9:43 AM
To: Bonnie Whitaker; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Processing/embedding question - your opinion

Bonnie:
  That is my personal and very particular opinion, and I think I am entitled to that,
  in the same way that you have expressed yours.
  I believe that this is covered by the freedom of expression, is it not?
  René J.

Bonnie Whitaker <bwhitaker <@t> brownpathology.com> wrote:
  Rene,

What is "lazy and indolent" about saving processed tissue without embedding
it? I frequently collect many tissues for embedding in multi-tissue blocks,
and I see no point in embedding the tissue temporarily while I continue to
collect additional material. It makes no difference if the paraffin coating
is minimal or a full block. You can also store a lot more control tissue in
a small area if it is maintained in unembedded cassettes. One cassette can
hold several blocks worth of material.

Bonnie Whitaker
Lab Manager
Brown & Associates Medical Laboratories
8076 El Rio
Houston, Texas 77054
713-741-6677

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, January 06, 2006 10:58 AM
To: foley1; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing/embedding question - your opinion


Hi Foley:
I have seen that done in several places. I have also seen all the
cassettes wrapped in aluminum foil and kept that way (like a pizza
left-over).
I cannot tell you what would be the consequences for the morphology or
future tests.
Theoretically speaking probably this practice will not affect because the
tissue itself and all its components are supposedly embedded in paraffin
that will just solidify.
I personally do not like this to be done. For me, personally, it indicates
laziness, indolence and even "disrespect" for the tissue sample.
Once I was confronted with the need of keeping processed cassettes in a
secure way before casting the blocks. My solution was to put all the
cassettes in a shallow
plastic container, place all the blocks in it, add melted paraffin and
prepare one single block, as large as the container. When I was able to
prepare the blocks individually, I melted the paraffin and prepared the
blocks.
Hope this will help you!
René J.

foley1 wrote:
Does anyone routinely allow for the hot wax to drain off multiple
cassettes of processed tissue and be held at room temperature for multiple
days (6
days) before embedding? What would the consequences be to morphology,
possible immunohistochemistry and molecular (DNA) studies?



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