[Histonet] optimal thickness for cutting of IHC sections

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Jan 3 10:12:16 CST 2006

  A 5 µm thichness is the general standard for most laboratories and we used it, BUT
  (there is always a "but" isn't it) if we were targeting a nuclear epitone we  tried a 
  little thicker (6-7 µm) and for cytoplasmic ones we tried somewhat thinner (ca. 4 µm).
  This approach was also used for histochemistry procedures: at least 6-7 µm for
  reticulum staining in bone marrows.
  I hope this will help you!
  René J.

"Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov> wrote:
  Hi Histonetters,

Is 5 microns the norm for IHC sections? What is considered optimal?



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