[Histonet] Uneven Staining

[Mary Parker]

Can anyone tell me if they have had uneven staining (H&E) on a linear
stainer?

 

This one is relatively new to our lab and we are having a very trying
time especially with liver, brain and heart sections.

 

We have done all our usual problem solving and ruled out fixation,
improper de-paraffinization, and microtoming artifacts.

 

The pattern is variable, inconsistent and random. When we use the same
staining set up and do the slides by hand we get better results.

 

Any suggestions would be appreciated.