[Histonet] H&E staining

[James Watson]

Try looking at the AFIP staining manual  "Laboratory methods in
Histotechnology" Uses eosin-Phloxine for 2 minutes then two minutes each
for all the alcohols and xylenes, very consistent , very easy, with
excellent staining.

James Watson HT, ASCP
Facilities Manager of Histology
GNF, Genomics Institute of the Novartis Research Foundation
Room C015
858-332-4647
jwatson <@t> gnf.org 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Malcolm
McCallum
Sent: Monday, February 27, 2006 9:47 AM
To: Massimo; eileen dusek
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E staining


Doesn't Hummason's Histotechnique manual have this in it?  Not
attacking, just trying to provide a helpful reference!  
 
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-233-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Massimo
Sent: Mon 2/27/2006 11:40 AM
To: eileen dusek
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] H&E staining



After Eosin:

10 - 15 seconds in distillate water
Quick steps for 50, 75, 95%  ETOH (they remove Eosin)
About 20 - 30 seconds for 100% ETOH ( for each step of two. You could
stop longer in this alcohol than the other ones above)

Pay attention!!! That procedure is coming from me: an amateur
naturalist. I think it would be better for you to lissen the opinions of
the "guru" Histologysts of this ML too!!!

Best Regards,

Massimo

----- Original Message -----
From: "eileen dusek" <eileen_dusek <@t> yahoo.com>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 27, 2006 5:30 PM
Subject: [Histonet] H&E staining


> Hi Everyone,
>  This is a basic question.
>  How long are tech leaving slides in 95% and 100% ETOH after eosin. I 
> feel we are in too long but would like to get other ideas.  Our slides

> have been very washed out, little to no contrast.
>
>  I appreciate your help
>
>  Eileen C. Dusek
>  Edward Hospital
>
>
> ---------------------------------
> Yahoo! Mail
> Bring photos to life! New PhotoMail  makes sharing a breeze. 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet