[Histonet] staining collagen

[Barbara Bublava]

hi Marc,

pikro-aniline blue:

add 0,5g Anilinblue or methylenblue to 500ml  saturated aquous solution of
picric acid.
everything else is done like van Gieson
Result: collagen, basement membranes and reticulin blue; cytoplasm yellow

John Kiernan´s book says reticulin and basement membranes are not stained if
you use indigocarmine (0,5 g in 200ml) collagen should be blue-green in this
case

greetings
Barbara

----- Original Message ----- 
From: "Marc Tjwa" <marc.tjwa <@t> med.kuleuven.be>
To: "Barbara Bublava" <barbara.bublava <@t> meduniwien.ac.at>
Sent: Sunday, February 26, 2006 4:32 PM
Subject: Re: [Histonet] staining collagen


Dear Barbara

I am also interested in the protocol that you
have suggested. Would you please be so kind to
send me the protocol by mail?

Tnx in advance

YS

Marc Tjwa




>You could use anilineblue instead of acid fuchsin. makes better contrast
>and is described in John Kiernans book
>
>tell me if you can´t find a protocol
>
>greetings
>Barbara, Vienna
>----- Original Message -----
>From: "Lobke De Bels" <Lobke.DeBels <@t> UGent.be>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Sent: Friday, February 24, 2006 8:58 AM
>Subject: [Histonet] staining collagen
>
>
>
>Von Gieson gives a good contrast for the human eye, but the computer can't
>make
>a difference between yellow/orange and red.
>If I only use acid fushine (or aniline) everything stains pink(blue).
>
>I have to find a way to stain the connective tissue so the computer can
>clearly
>make a difference between connective tissue and the other tissue.
>
>The ideal should be a method that only stains collagen.
>
>
>Many thanks in advance for any help
>
>
>
>Lobke De Bels
>Department of Morphology
>Faculty of Veterinary Medicine
>Ghent University
>Salisburylaan 133
>9820 Merelbeke
>Belgium
>lobke.debels <@t> ugent.be
>


-- 
============================================================
Marc Tjwa, MD
Center for Transgene Technology and Gene Therapy (CTG)
Flanders Interuniversity Institute for Biotechnology (VIB)
University of Leuven (KUL)
Campus Gasthuisberg, Herestraat 49
B-3000, Leuven, Belgium
Tel: +32-16-345775; Fax: +32-16-345990
E-mail: Marc.Tjwa <@t> med.kuleuven.be
============================================================

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