[Histonet] staining collagen

[Barbara Bublava]

You could use anilineblue instead of acid fuchsin. makes better contrast
and is described in John Kiernans book

tell me if you can´t find a protocol

greetings
Barbara, Vienna
----- Original Message ----- 
From: "Lobke De Bels" <Lobke.DeBels <@t> UGent.be>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, February 24, 2006 8:58 AM
Subject: [Histonet] staining collagen



Von Gieson gives a good contrast for the human eye, but the computer can't
make
a difference between yellow/orange and red.
If I only use acid fushine (or aniline) everything stains pink(blue).

I have to find a way to stain the connective tissue so the computer can
clearly
make a difference between connective tissue and the other tissue.

The ideal should be a method that only stains collagen.


Many thanks in advance for any help



Lobke De Bels
Department of Morphology
Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133
9820 Merelbeke
Belgium
lobke.debels <@t> ugent.be


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