[Histonet] Microwave processing

[Bartlett, Jeanine]

We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains.  So far the tissues form the Xpress have been as good as, and is some cases superior to, the traditional processing method.
 
Jeanine Bartlett
CDC Atlanta

	-----Original Message----- 
	From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of John PJ Coleman 
	Sent: Wed 2/22/2006 5:04 PM 
	To: histonet <@t> lists.utsouthwestern.edu 
	Cc: 
	Subject: [Histonet] Microwave processing
	
	

	I am the Senior tech of a large hospital corporation.  My
	administration has just won funding for 4 Sakura Microwave rapid
	processing units. We run FISH her 2 on formalin fixed paraffin embedded
	tissue as per FDA protocol. As I tech, I am not in favor of tossing
	routine processing wholesale in favor of a completely new technology
	without thorough testing and parallel processing. Also, we are a
	regional reference lab for IHC and have a panel of 115 antibodies, all
	optimized for formalin fixed paraffin embedded tissue. We run an
	average of 350 IHC slides a day, max 580 per day and would have to
	re-optimize these to use in the new formalin free world while keeping
	our FFPE procedures in parallel for our reference lab work. Much like
	running 2 labs. If anyone has any insight, or if anyone currently uses
	these instruments for routine and/or IHC, feel free to call or email,
	and I'll check the postings on this string. We are also taking
	invitations to come out and see these things in use real time.
	
	John PJ Coleman-757 335-2159
	http://members.cox.net/captainmadman/
	http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/
	
	
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