[Histonet] Microwave processing

[Rene J Buesa]

John:
  I know this instrument and the technology is safe. You will not need to do HIER for IHC since the tissue will be fixed in an alcohol based fixatives and I don't think that you will need to run parallel runs.Maybe you will have to dilute your antibodies a little more than for formalin + HIER.
  The turn around time is 120 cassettes per hour, and with 4 of these instruments (which I personally think is a "bit too much") what you will have to do is to rethink your whole staff scheduling.
  Try to get a book titled:
  Microwaves for the Art of Microscopy by L.P Kok and M.E.Boon, Coulomb Press Leyden 2003 (you can get it at http://www.coulomb.nl
  This instrument is ideal for a large volume reference lab like yours.
  Hope this will help you.
  René J.

John PJ Coleman <jcolclefa <@t> aol.com> wrote:
  I am the Senior tech of a large hospital corporation. My 
administration has just won funding for 4 Sakura Microwave rapid 
processing units. We run FISH her 2 on formalin fixed paraffin embedded 
tissue as per FDA protocol. As I tech, I am not in favor of tossing 
routine processing wholesale in favor of a completely new technology 
without thorough testing and parallel processing. Also, we are a 
regional reference lab for IHC and have a panel of 115 antibodies, all 
optimized for formalin fixed paraffin embedded tissue. We run an 
average of 350 IHC slides a day, max 580 per day and would have to 
re-optimize these to use in the new formalin free world while keeping 
our FFPE procedures in parallel for our reference lab work. Much like 
running 2 labs. If anyone has any insight, or if anyone currently uses 
these instruments for routine and/or IHC, feel free to call or email, 
and I'll check the postings on this string. We are also taking 
invitations to come out and see these things in use real time.

John PJ Coleman-757 335-2159
http://members.cox.net/captainmadman/
http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/


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