[Histonet] Re: blue mucin

[Lee & Peggy Wenk]

Check the pH. We had this problem before (different vendor). New hematoxylin
out of the bottle had a pH about 2.7, and with water carry would quickly get
up to 3.0.

Add acetic acid, until pH is 2.4-2.5

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
LaFriniere
Sent: Monday, February 13, 2006 1:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: blue mucin

Any help would be appreciated....
 
I am noticing recently on routine H&E sections, the mucin on colon biopsies
only, demonstrating a strong blue color....I know the mucin is picking up
the hematoxylin stain and not differentiating in the acid rinse. I was
wondering if anybody has demonstrated this recently and the possible
corrections to end this aggravation. This leads me to believe that it may be
something with the patient prep prior to the biopsy? I am using the Richard
Allen (7221) Hematoxylin and routine reagents.  Any suggestions would be
greatly welcomed!
 
Thank you
 

                                        


 
Michael R. LaFriniere
Executive Director
Cytology Services of Maryland (CSM)
301-206-2555 ext 27
301-206-2595 fax
michael.lafriniere <@t> csmlab.com



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