[Histonet] stain

[Chris Pomajzl]

Could also be inadequate de-paraffinization.

----- Original Message ----- 
From: "Kemlo Rogerson" <kemlo.rogerson <@t> waht.swest.nhs.uk>
To: "'Paula Hineline'" <paulah <@t> grandpath.com>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, February 14, 2006 2:42 AM
Subject: RE: [Histonet] stain


> 'Milky' nuclear detail could be poor fixation, however it's patchy so
that's
> odd. It could be water in the final clearing agent, it could be
contaminated
> mounting medium (maybe water again), it could be carry over of water from
> contaminated alcohol to the clearing agent.
>
> Used to have this and poor resolution that was finally traced to poor
> fixation; which oddly can be patchy. Contaminated clearing agent or those
> fast drying mounting mediums also can cause poor resolution due to the
> scattering of light. Try taking the coverslip off, rehydrate and dehydrate
> with fresh alcohols and clearing agent; see if it goes away. If it doesn't
> then fresh mounting medium and if that fails, post fix the sections and
> restain.
>
> Kemlo Rogerson
> Pathology Manager
> Ext  3311
> DD   01934 647057
> Mob 07749 754194
>
>
>
>
> -----Original Message-----
> From: Paula Hineline [mailto:paulah <@t> grandpath.com]
> Sent: Monday, February 13, 2006 8:06 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] stain
>
> We are seeing some problems with our short-run / small biopsy H&E
> stained slides. The nuclear detail varies from good to "milky" and
> indistinct. Nuclear detail can be markedly indistinct with variations
> occurring from slide to slide and even on the same slide. We are in the
> process of troubleshooting but I am hoping someone might recognize this
> problem and have some suggestions. Your responses are much appreciated.
>
> Paul Wozniak
> Laboratory Manager
> Pathology Laboratory
> Grandville, Mi
> 616.530.3344 x 101
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