[Histonet] plastic embedded bone sections

[Michael J Voor]

Dear Histonet,

We are trying to section PMMA (Technovit 9100 New) embedded
undecalcified bone samples (rat tibias).  We plan to use a Microm
sliding microtome.  We need advice on exactly what blade to use and what
blade angle would be best to create sections on the order of 7-10
microns.  Thanks for any help.


Michael J. Voor, Ph.D.
Orthopaedic Bioengineering Laboratory
Director of Research
Department of Orthopaedic Surgery   
University of Louisville
Louisville, KY  40292
(502) 852-7673  FAX: (502)852-7227
e-mail: mike.voor <@t> louisville.edu