[Histonet] Technovit 7100 resin removal

[Patsy Ruegg]

True Gayle, there are techniques to etch or soften the GMA but it cannot be
dissolved.  We used to use a very strong ethoxide (alcohol in sodium
hydroxide) solution to etch before staining (this method does not fair well
for IHC).  As you mentioned, the best bet to do IHC is to use MMA.  
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, February 07, 2006 1:37 PM
To: germckeon <@t> excite.com; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Technovit 7100 resin removal

Techovits 7100 is a Glycol methacrylate (GMA)  formulation and once 
polymerized, cannot be removed from the section or etched either.   Water 
may soften the plastic but it does NOT dissolve it away.   If you want to 
remove a plastic from a section for Immunostaining, then the Technovits
9100, methyl methacrylate would be a better choice for embedding, then use
60C xylene or a solvent that will dissolve the MMA away.

   At 10:52 AM 2/7/2006, you wrote:





>Hello Jorge,
>
>I am also trying to find out the same thing. Would it be possible to 
>send me any hints you may get?
>
>
>
>Many thanks,
>
>Gerald McKeon
>
>Trinity College,
>
>Dublin, Ireland.
>
>
>
>
>
>[Histonet] Technovit 7100 resin removal
>
>
>
>Hi all,
>
>
>
>I would like to remove the resin in my slides prior to stain. Do you
>
>know any protocol to do this?
>
>
>
>Thank you very much in advance
>
>
>
>Jorge Tornero
>
>IEO Cádiz - Spain
>
>
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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