[Histonet] Technovit 7100 resin removal

[Gayle Callis]

Techovits 7100 is a Glycol methacrylate (GMA)  formulation and once 
polymerized, cannot be removed from the section or etched either.   Water 
may soften the plastic but it does NOT dissolve it away.   If you want to 
remove a plastic from a section for Immunostaining, then the Technovits 
9100, methyl methacrylate would be a better choice for embedding, then use 
60C xylene or a solvent that will dissolve the MMA away.

   At 10:52 AM 2/7/2006, you wrote:





>Hello Jorge,
>
>I am also trying to find out the same thing. Would it be possible to send 
>me any hints you may get?
>
>
>
>Many thanks,
>
>Gerald McKeon
>
>Trinity College,
>
>Dublin, Ireland.
>
>
>
>
>
>[Histonet] Technovit 7100 resin removal
>
>
>
>Hi all,
>
>
>
>I would like to remove the resin in my slides prior to stain. Do you
>
>know any protocol to do this?
>
>
>
>Thank you very much in advance
>
>
>
>Jorge Tornero
>
>IEO Cádiz - Spain
>
>
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)