[Histonet] Immunohistochemistry on mouse teeth

[Amy Porter]

Diane - Are you using a mouse primary?  Didn't make a comment about this in 
your original post.  If you are you would want to be using mouse on mouse 
staining techniques or kits.  We always use another piece of tissue from the 
same animal if possible as a postive control, usually something from G.I. 
tract.  Lots of proliferation going on there.  Hope this helps out,
----- Original Message ----- 
From: "Horn, Diane A" <hornd <@t> uthscsa.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Sunday, February 05, 2006 11:19 PM
Subject: [Histonet] Immunohistochemistry on mouse teeth



I am currently doing IHC on develping mouse teeth and I am getting only 
backgroung staining.  The tissues are fixed O/N in 4% paraformaldehyde @4 
degrees and embedded in paraffin.  I have cut my sections @ 4um.  I have 
tried HIER with Vector unmasking solution (boiling) for 2 minutes and using 
a kit for PCNA from Zymed.  Has anyone done IHC on teeth that could give me 
some advice on what to try next?

Thanks!
Diane


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