[Histonet] paraffin embedding of frozen tissue

[Carmen Contreras-Sesvold]

Greetings histonetters,
I have recently found that sectioning frozen lung is a bit difficult. I was wondering if  anyone has ever paraffin embedded previously fixed (and cryproserved) frozen sections? And if  so what were the results? I am performing immunohistochemistry / immunofluorecent staining on mice lung tissue and I was just wondering if it was possible.  I also encourge advice on the subject matter.


I have learned quite a bit from this web site.
Thank you very much.
Carmen Contreras-Sesvold