[Histonet] paraffin "stuff"???

[Jackie M O'Connor]

There always seems to be some kind of dirt in paraffin, no matter who the 
manufacturer is - it's not created in a sterile environment by any means. 
Dirt or dust is bound to get in during the manufacturing/packaging 
process.  (Did you ever check to see how many insect parts are allowed by the FDA in a jar of baby applesauce?)   Anyway, you've got a screen 
filter in the bottom of your embedding center to filter out the chunks, 
right?  There is always some "dark stuff" when you wipe that tank out from 
time to time, right?  Dirt.  If it gets in your block during embedding, 
you'll know by the unexplained scratches in your sections.  I've never 
known this cosmic dust to create any real problems during 
processing/infiltration - sometimes the gunk you wipe out of the 
infiltration pots are carryover from all the processing, little 
collections of tissue debris and the like.  Just keep your equipment clean 
and wipe that stuff out when you see it - and check out the stuff about 
bugs in baby food, that's WAY more interesting.   All processed food is 
allowed a certain amount of foreign matter before it's rejected - and that 
IS supposed to be a much cleaner manufacturing process.  The moral of the 
story - grow your own.  (I don't - my mantra is whatever doesn't kill us 
makes us stronger.)
Jackie O'




"Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/02/2006 02:14 PM

 
        To:     "Steven Coakley" <sjchtascp <@t> yahoo.com>, Histonet <@t> lists.utsouthwestern.edu
        cc:     (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT)
        Subject:        RE: [Histonet] paraffin "stuff"???


Check the temperature of the paraffin.  It could be bits of the plastic 
component not melting in solution - just existing on a borderline 
temperature.               -Janet

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Steven 
Coakley
Sent: Thu 2/2/2006 12:22 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] paraffin "stuff"???



I've noticed what appears to be this off colored "fuzzy"  stuff floating 
in the paraffin thats in my embedding dispencer.  I drained the dispencer 
and replaced it with fresh but there it is again.  Is this anything to be 
concerned about.  "???"  maybe i should check the processors paraffins. 
Never noticed this "stuff" before.

 
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