[Histonet] Active caspase 3 staining of mouse small intestine

[John C. Dennis]

I'm preparing a Caspase 3 preparation today, in fact.  The tissue is 
paraffin embedded rat ethmoturbinate.  I boil the slides in 10 mM Na 
Citrate 
pH 6.0 20'.  I'm using Sigma's rabbit polyclonal and Vector's DAB kit.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Wed, 1 Feb 2006, Yu, Jian wrote:

> Thanks to several of you who have given me a lot of great suggestions on
> how to get good cross sections of mouse small intestine   One of you
> mentioned that you do Caspase 3 staining routinely with the small
> intestine, sorry that I could not find your email anyone.  I have been
> using the CAM1 Ab from BD, which works well for IF on frozen sections
> but have a lot of background for IHC.  Unfortunately the frozen sections
> do not have the greatest structures.  I would very much appreciate that
> if you could share your experience with paraffin sections.
>
>
>
> Thanks again!
>
> ********************************************************
>
> Jian Yu, Ph. D.
>
> University of Pittsburgh Cancer Institute
>
> Hillman Cancer Center Research Pavilion
>
> Office suite 2.26h, Laboratory 2.43
>
> 5117 Centre Avenue, Pittsburgh, PA 15213
>
>
>
> Phone : 412-623-7786, (Lab) 412-623-3255
>
> Fax:      412-623-7778
>
> Email:   yuj2 <@t> upmc.edu
>
> ********************************************************
>
>
>
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