[Histonet] Section adhesion to slides

Rene J Buesa rjbuesa <@t> yahoo.com
Thu Dec 28 11:03:13 CST 2006

For really difficult sections (decals, toe nails, poorly infiltrated breasts and the likke) I used 2 methods (besides the "conventional" + charged slides):
  1- coat the slides with a 5% Elmer's glue solution; let the slides dry and use for the sections the next day (or when needed); or
  2- add 1 mL of Elmer's glue to a regular 2 litres water bath (to make a 0.05% solution) and use it as your  regular water bath (in case you have many difficult cases). The water will have a "milky" appearance, but that is OK
  Elmer's glue does not interfere with any regular or special stains, nor with IHC procedures.
  René J.

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