[Histonet] mammary gland embedding

[pruegg <@t> ihctech.net]

I agree with Paul on this, I cannot see any reason to embed mammary tissue,
animal or human as cross-section.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Paul
Sent: Thursday, December 14, 2006 2:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] mammary gland embedding

I can't think of any reason to view such a flat, essentially homogeneous
organ in cross section.  The glandular structure is random in organization,
not restricted to any particular plane .  IMHO, the only effect of using the
cross sectional view is that you will see far less of the tissue, and be far
more likely to miss any localized pathology - unless you do step sections
all the way through, and I see no point in that.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Till,
Renee
> Sent: 	Thursday, December 14, 2006 12:38 PM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] mammary gland embedding
> 
> I work with a lot of rat mammary gland tissue and depending on the age,
the
> whole gland or most of it will usually lay flat and fit in the cassette.
We
> recently had it suggested that we might try doing cross sections instead.
Why
> would I do that? Is there some reason looking at a cross section view
would
> be any different/better? The person who suggested it works with both human
> and animal tissue, so I thought it might be something more commonly done
with
> human tissue where you are just taking a piece rather than the whole
thing. I
> feel like this is one of those things I should know, but I just don't
> remember anything about embedding mammary tissue different ways. Thanks.
> 
>  
> 
> Renee' Till, HT
> 
> Research Assistant
> 
> Arkansas Children's Nutrition Center
> 
> 1212 Marshall St./N2021
> 
> Little Rock, AR 72202
> 
> Office (501)364-2785
> 
> Fax (501)364-3161
> 
>  
> 
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