[Histonet] mammary gland embedding

[Monfils, Paul]

I can't think of any reason to view such a flat, essentially homogeneous organ in cross section.  The glandular structure is random in organization, not restricted to any particular plane .  IMHO, the only effect of using the cross sectional view is that you will see far less of the tissue, and be far more likely to miss any localized pathology - unless you do step sections all the way through, and I see no point in that.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Till, Renee
> Sent: 	Thursday, December 14, 2006 12:38 PM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] mammary gland embedding
> 
> I work with a lot of rat mammary gland tissue and depending on the age, the
> whole gland or most of it will usually lay flat and fit in the cassette. We
> recently had it suggested that we might try doing cross sections instead. Why
> would I do that? Is there some reason looking at a cross section view would
> be any different/better? The person who suggested it works with both human
> and animal tissue, so I thought it might be something more commonly done with
> human tissue where you are just taking a piece rather than the whole thing. I
> feel like this is one of those things I should know, but I just don't
> remember anything about embedding mammary tissue different ways. Thanks.
> 
>  
> 
> Renee' Till, HT
> 
> Research Assistant
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> Arkansas Children's Nutrition Center
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