[Histonet] Methylmethacrylate to LR White?

[pruegg <@t> ihctech.net]

Matt,
Methyl methacrylate can be removed with xylene, but glycol methacrylate can
not ever be completely removed, I believe LR White is a glycol methacrylate.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of matt
prideaux (RI)
Sent: Friday, December 15, 2006 5:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Methylmethacrylate to LR White?

Hi all,

I need to remove the methacrylate surrounding some bone samples (I was
told that a 50:50 solution of xylene and chloroform would do the trick).
The problem is that I want to re-embed the samples in LR White once the
methacrylate has been removed. Does anyone have any experience in this
or knows if it is possible? I need to cut 10 micron thick cross-sections
of mouse tibiae and these have failed to adequately infiltrate with
methacrylate. With LR White being less viscous I was hoping that it
would infiltrate better.

Thanks

Matt

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