[Histonet] Methylmethacrylate to LR White?
matt prideaux (RI)
matt.prideaux <@t> bbsrc.ac.uk
Fri Dec 15 06:02:25 CST 2006
Hi all,
I need to remove the methacrylate surrounding some bone samples (I was
told that a 50:50 solution of xylene and chloroform would do the trick).
The problem is that I want to re-embed the samples in LR White once the
methacrylate has been removed. Does anyone have any experience in this
or knows if it is possible? I need to cut 10 micron thick cross-sections
of mouse tibiae and these have failed to adequately infiltrate with
methacrylate. With LR White being less viscous I was hoping that it
would infiltrate better.
Thanks
Matt
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