[Histonet] mosquito section on floatation bath

[Monfils, Paul]

While I can't explain the difference between the infected and non-infected mosquitos, such exposion usually indicates poor infiltration with paraffin - which would also be hard to explain if all the specimens were processed together.  In any case, this problem can often be avoided by adding a small drop of liquid dish detergent to the water bath to reduce surface tension. (detergent made for hand washing of dishes, not dishwasher detergent)

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Helen E Johnson
> Sent: 	Wednesday, December 13, 2006 8:49 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] mosquito section on floatation bath
> 
> 
> Hi Histonetters,
>       I am having a problem when I place FFPE mosquito sections on the
> floatation bath.  The abdomen explodes.  This is happening only with
> experimentally infected mosquitoes and not with noninfected mosquitoes. I
> tried lowering the temperature of the floatation bath.  Any suggestions?
> Helen Johnson  (hej01 <@t> health.state.ny.us)
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
>