[Histonet] Rapid freezing using n-Pentane

Gregory A. O'Sullivan osullivan <@t> mpih-frankfurt.mpg.de
Mon Dec 11 11:53:19 CST 2006

Dear Histonetters,

Could anyone give a reference or a protocol for the rapid freezing of
postnatal P1 brains from rodents?
We require such a protocol in order to minimise the amount of shearing that
is observed by rapid freezing on dry-ice.

Many thanks,


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