ree3 <@t> leicester.ac.uk
Mon Dec 11 11:14:55 CST 2006
Often referred to as "squams", also derived from flaky scalps, i.e. dandruff!!.
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: 11 December 2006 16:56
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] floaters
Yes, we all have had the problem at one time or another - excellent points about friable tissue. I think one reply on Histonet, the tech used newpaper to skim the waterbath surface. Make sure the paper is not overly fibrous paper, you don't need shreds of paper on top of your sections either.
One of the most annoying floaters comes from the microtomist. Epidemal cells from the skin exfoliate into the waterbath especially when you allow fingers to dip into the water, and then stain very nicely with the H&E, generally perched on your beautiful section. Grip the slides on the edge
or very ends to avoid this. Pencil "lead" or graphite exfoliates black
blobs on tissue sections, use a slightly harder lead, buy a slide labeler, or use a permanent pen designed for labeling glass slides/cassettes.
René brings up a good point about dirty forceps/heated forcep wells on embedding centers. At one clinical lab, we wiped the forceps between each block before returning to the well, and cleaned the wells daily. We still saw the annoying skin cells though, so learn to not go 'fingerdipping" in the water bath.
Research Histopathology Supervisor
Veterinary Molecular Biology
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