[Histonet] Polymer Feedback?

[patsy ruegg]

Bonnie,
You are generous, my boss always said "pick one" out of the three you
mentioned.
In the case of labeled polymer I think you are right, they are faster and I
think better, at least I do not have to worry about endogenous biotin
issues.  They an't cheap though.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bonnie
Whitaker
Sent: Thursday, December 07, 2006 12:07 PM
To: 'Dawson, Glen'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Polymer Feedback?

Like a boss used to tell me:  You can have better quality, you can have a
faster turnaround time or you can produce a product that is cheaper.  Pick
2.  You can never have all three!

Polymers are faster and better (my favorite pick for the 2)

Bonnie

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson, Glen
Sent: Thursday, December 07, 2006 12:25 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Polymer Feedback?


Doug,

Polymers Rock!!!  No avidin/biotin blocking.  Better sensitivity so you save
some of their extra cost by taking antibody titers out farther.  Clean.
Fewer steps.  So on and so on.

Only bad thing is the cost but nothing is perfect.

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Doug Geddes
Sent: Thursday, December 07, 2006 11:48 AM
To: Histonet <@t> lists.utsouthwestern.edu; Gayle Callis
Subject: Re: [Histonet] Polymer Feedback?


Sorry, polymers for IHC staining.

Doug

>>> Gayle Callis <gcallis <@t> montana.edu> 2006-12-07 12:36 PM >>>
Doug,

Polymers for what purpose, embedding tissues?

At 09:22 AM 12/7/2006, you wrote:
>Dear Histonet,
>
>Looking for feedback and experiences with different ploymers 
>(preferably universal) in a clinical setting.
>
>Doug Geddes BSc, MLT
>Dept of Pathology
>LHSC London ON
>Canada

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717


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