[Histonet] paraffin problem

Douglas D Deltour doug <@t> ppspath.com
Thu Dec 7 09:16:45 CST 2006


I have been through many types of paraffin. I now use Paraplast plus for
infiltration and the Paraplast xtra for embedding. The xtra is the best
ribboning paraffin that I have found. There may be others comparable but I
do not have the time to try any more out. 

Douglas D. Deltour HT(ASCP)
Histology Supervisor
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Fax (803)254-3262
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Thursday, December 07, 2006 9:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] paraffin problem


Lately we are experiencing a problem with our paraffin blocks.  We use
Richard Allen Type 9 and Type 1.   The tissue doesn't seem to ribbon
very well and at times the sections have some small striations, almost
like knife lines.  We have checked the disposable knives and have
eliminated that variable.  We use a recycled clearing agent but have
eliminated that also by using fresh chemicals.  We have several tissue
processors and there is a possibility that the problem could be a
processor issue however the majority of us think that it is a paraffin
problem.  We do not see any "grit" in the paraffins.  We are thinking of
trying a different paraffin and wondered if anyone has switched to one
of the newer paraffins that work great.  Obviously I can get as many
samples as I would like but want to see if anyone has any suggestions or
ideas that would help us eliminate some of the steps of the process.


Jim Vickroy

Technical Supervisor - Surgical and Autopsy Pathology

Memorial Medical Center


This message (including any attachments) contains confidential information
intended for a 
specific individual and purpose, and is protected by law. If you are not the
intended recipient, 
you should delete this message. Any disclosure, copying, or distribution of
this message, or the 
taking of any action based on it, is strictly prohibited.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list