[Histonet] paraffin problem

[Douglas D Deltour]

Jim,

I have been through many types of paraffin. I now use Paraplast plus for
infiltration and the Paraplast xtra for embedding. The xtra is the best
ribboning paraffin that I have found. There may be others comparable but I
do not have the time to try any more out. 

 
Douglas D. Deltour HT(ASCP)
Histology Supervisor
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Thursday, December 07, 2006 9:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] paraffin problem

 

Lately we are experiencing a problem with our paraffin blocks.  We use
Richard Allen Type 9 and Type 1.   The tissue doesn't seem to ribbon
very well and at times the sections have some small striations, almost
like knife lines.  We have checked the disposable knives and have
eliminated that variable.  We use a recycled clearing agent but have
eliminated that also by using fresh chemicals.  We have several tissue
processors and there is a possibility that the problem could be a
processor issue however the majority of us think that it is a paraffin
problem.  We do not see any "grit" in the paraffins.  We are thinking of
trying a different paraffin and wondered if anyone has switched to one
of the newer paraffins that work great.  Obviously I can get as many
samples as I would like but want to see if anyone has any suggestions or
ideas that would help us eliminate some of the steps of the process.
Thanks.

 

Jim Vickroy

Technical Supervisor - Surgical and Autopsy Pathology

Memorial Medical Center

 



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