[Histonet] paraffin problem

[Vickroy, Jim]

Lately we are experiencing a problem with our paraffin blocks.  We use
Richard Allen Type 9 and Type 1.   The tissue doesn't seem to ribbon
very well and at times the sections have some small striations, almost
like knife lines.  We have checked the disposable knives and have
eliminated that variable.  We use a recycled clearing agent but have
eliminated that also by using fresh chemicals.  We have several tissue
processors and there is a possibility that the problem could be a
processor issue however the majority of us think that it is a paraffin
problem.  We do not see any "grit" in the paraffins.  We are thinking of
trying a different paraffin and wondered if anyone has switched to one
of the newer paraffins that work great.  Obviously I can get as many
samples as I would like but want to see if anyone has any suggestions or
ideas that would help us eliminate some of the steps of the process.
Thanks.

 

Jim Vickroy

Technical Supervisor - Surgical and Autopsy Pathology

Memorial Medical Center

 



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