[Histonet] Re: Thermostat for Tissue Tek II Embedding Center

Billing Consultants, LLC billingconsultants <@t> yahoo.com
Sun Dec 3 12:09:35 CST 2006

A good try would be Analytical Instruments.  They are always very helpful. Their website is www.aibltd.com. 
  Louri Roberts
  Billing Consultants, LLC
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Today's Topics:

1. Thermostat For Tissue Tek II Embedding Center (Marilyn Johnson)
2. Tube cross sections in Frozens (Donna Harclerode)


Message: 1
Date: Sat, 2 Dec 2006 12:34:38 -0700
From: "Marilyn Johnson" 
Subject: [Histonet] Thermostat For Tissue Tek II Embedding Center
Message-ID: <001901c71648$e9991a90$6801a8c0 <@t> VALUED20606295>
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonetters,
I am looking for a thermostat for the upper compartment of a Tissue Tek II embedding center, Model 4603. Any replies would be greatly appreciated.
Thank you in advance.

Marilyn Johnson
Edmonton, AB. Canada


Message: 2
Date: Sat, 2 Dec 2006 20:35:04 -0800
From: "Donna Harclerode" 
Subject: [Histonet] Tube cross sections in Frozens
Cc: TillRenee <@t> uams.edu
<3DE0F644E093DF4BAE80C254176696A5013E9E <@t> mp-mailserver.macropore.com>
Content-Type: text/plain; charset="iso-8859-1"

Hi Renee'

I multiple embed rat (and mouse) gi in Peel Away® embedding molds. I embed laying down, freeze and then cut the blocks on end.
I put a bit of OCT in then arrange however many piecies I want in the block, then add more OCT until completely covered and recheck to see if any moved too much and then freeze. I line the tubes up to cut where the 2 dimples are in the molds (or you could mark the mold with a Sharpie) so I know which side to trim to get the correctt orientation. I can easily put 3-5 in a 22x22 or 22x30mm blocks. I end up with a very deep block (22mm) when I turn them on the end, but so far all my cryostats have not had probems doing this. I usually leave a piece of pancreas on the duodenum and put them in in order so I can tell which piece is which when they are in the blocks without a scope. 

Good luck

Donna Harclerode HT(ASCP) HTL, QIHC
Cytori Therapeutics
9020 Callan Rd
San Diego, A 92021

heMessage: 5
Date: Fri, 1 Dec 2006 13:25:20 -0600
From: "Till, Renee" 
Subject: [Histonet] positioning tissues in OCT
To: histonet <@t> lists.utsouthwestern.edu
<11F927674DEBDC43B960809A7403C5D202A9E44E <@t> MAILPED.ad.uams.edu>
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For our paraffin blocks we embed rat gi on end so when sectioned you get
a little circle of tissue. Any suggestions on how to do this for
cryosections? The fresh tissues have been too soft and flexible to stay
standing up in the OCT until it freezes. Thanks.

Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72202

Office (501)364-2785

Fax (501)364-3161


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