[Histonet] Her2Neu

Patti Loykasek ploykasek <@t> phenopath.com
Fri Dec 1 16:22:24 CST 2006

Hi Dorothy. I agree with some of the other posts that if you are using an
FDA approved Her2 kit you should comply with the kit protocol which usually
includes recommended fixation.
We have noticed in our practice that alcoholic formalin increases the signal
in our Her2 IHC. I would recommend that you have a subset of your IHC Her2
cases tested with Her2 FISH and see what your correlation is. This would
give you an idea if your fixation is causing a false Her2 score.
I would also like to recommend that techs educate themselves on the upcoming
Her2 testing guidelines that ASCO & CAP will be implementing. The
pathologists need to be aware of these too.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

> Presently our pathologists only order Her2Neu off of the breast needle
> cores and not the excision or mastectomy.  Would others please share
> there scope of practice regarding this area.  Also, presently , we fix
> all breast tissue (other than needle cores or enblocs) in Davidson's and
> process with an alcoholic fixative from BBC.  Will this cause problems
> with Her2Neu interpretation and/or other IHC concerns?  Is there anyone
> else that uses an alcoholic formalin to process fatty tissue and has
> competent results with their IHC's?  I would appreciate any and all
> feedback on this issue!!
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