[Histonet] Her2Neu

Weems, Joyce JWEEMS <@t> sjha.org
Fri Dec 1 07:57:23 CST 2006

And if your patient were to be included in a clinical trial, the Her2 testing would be required to have been carried out following the FDA approved protocol, which calls for fixation in 10% buffered formalin. 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Rene J
Sent: Friday, December 01, 2006 8:53 AM
To: Webb, Dorothy L; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Her2Neu

We did Her2Neu on both needle and regular breast specimens.
  Theoretically any alcoholic fixative will not interfere with IHC; as a matter of fact they should interfere less than NBF.
  As interpretation goes I only foresee a problem because of the fact that probably the control slides you will use (if supplied by DAKO, for example) probably come from a culture not fixed in the same way as your specimen.
  In this respect you should ask for DAKO's imput.
  René J.

"Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
  Presently our pathologists only order Her2Neu off of the breast needle
cores and not the excision or mastectomy. Would others please share
there scope of practice regarding this area. Also, presently , we fix
all breast tissue (other than needle cores or enblocs) in Davidson's and
process with an alcoholic fixative from BBC. Will this cause problems
with Her2Neu interpretation and/or other IHC concerns? Is there anyone
else that uses an alcoholic formalin to process fatty tissue and has
competent results with their IHC's? I would appreciate any and all
feedback on this issue!!
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